Journal: Journal of Tissue Engineering

Article Title: Continuous nutrient supply culture strategy controls multivesicular endosomes pathway and anti-photo-aging miRNA cargo loading of extracellular vesicles

doi: 10.1177/20417314231197604

Figure Lengend Snippet: Characterization and production: comparison of EVs: (a) isolation method of EVs, (b) TEM images of EVs, (c) particles size distribution, (d) western blot analysis of the particles to detect the expression of EV protein markers (CD81, CD63, CD9 and TSG101), (e) protein concentration of EV suspensions, (f) the number of EV pellets derived from each cell, (g) EV particle concentration, and (h) the size distribution of CCEVs and SC-EVs. Data are expressed as mean plus and minus standard deviations ( n = 3), *** p < 0.001.

Article Snippet: Finally, the total protein extract (40 μg) was separated and the protein expression of Protein 1A/1B-light chain I/II (LC3 I/II), PARP (ZenBioScience, China), CD9, CD63 and CD81 (Abcam, UK) were examined according to the previous method.

Techniques: Comparison, Isolation, Western Blot, Expressing, Protein Concentration, Derivative Assay, Concentration Assay

Journal: Journal of Tissue Engineering

Article Title: Continuous nutrient supply culture strategy controls multivesicular endosomes pathway and anti-photo-aging miRNA cargo loading of extracellular vesicles

doi: 10.1177/20417314231197604

Figure Lengend Snippet: Differential transport of tetraspanins in PM affects the formation of precursors of EVs (Intracellular vesicles content is equal to the whole membrane content minus cells surface membrane content): (a) immunofluorescence flow cytometry of tetraspanins (CD9, CD63, CD81) in PM of SC-MSCs and (b) CCMSCs, (c) expression of tetraspanins in whole cell lysates, (d) quantitative analysis of gray values, (e) images of cell sections captured in different culture methods by TEM, the upper and lower scale bar separately represents 2 μm and 500 nm, and (f) the number of MVEs of MSCs cultured in different ways, the budding ILVs within each MVE, and the membrane surface area of each MVE. Data are expressed as mean plus and minus standard deviations, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Finally, the total protein extract (40 μg) was separated and the protein expression of Protein 1A/1B-light chain I/II (LC3 I/II), PARP (ZenBioScience, China), CD9, CD63 and CD81 (Abcam, UK) were examined according to the previous method.

Techniques: Membrane, Immunofluorescence, Flow Cytometry, Expressing, Cell Culture

Journal: Cell

Article Title: LXR/ApoE activation restricts innate immune suppression in cancer

doi: 10.1016/j.cell.2017.12.026

Figure Lengend Snippet: Percent tumor-infiltrating immune cells of total CD45+ tumor-infiltrating lymphocytes (TILs), A–D, in B16F10 tumors in mice treated with control or GW3965 (100 mg/kg) administered in chow when tumors reached 5–10 mm3 in volume. Flow cytometry analysis was performed 14 days after tumor injection (n = 6). (A) Foxp3+ T regulatory cells, (B) CSF-1R+ tumor-associated macrophages, (C) total granulocytic myeloid-derived suppressor cells, and (D) total myeloid lineage cells. Representative plots show CD11b+ Gr-1high granulocytic MDSC populations.

Article Snippet: Flow cytometry analysis of human blood samples was performed by Serametrix Corporation (Carlsbad, California).

Techniques: Control, Flow Cytometry, Injection, Derivative Assay